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This chapter discusses the preparation of carbohydrates for analysis by modern chromatography and electrophoresis. The sample preparation addresses: (1).
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Ziad El-Rassi. This book is an updated and expanded edition of Carbohydrate Analysis, High Performance Liquid Chromatography and Capillary Electrophoresis and is concerned with the analysis of carbohydrates by modern chromatography and electrophoresis including analytical and preparative high performance liquid chromatography HPLC , thin layer chromatography TLC , field flow fractionation FFF , capillary electrophoresis CE , capillary electrochromatography CEC , polyacrylamide gel electrophoresis PAGE , gas chromatography GC and supercritical fluid chromatography SFC. Thirty-one chapters cover: various modes of HPLC, CE, CEC, FFF, GC and SFC that are currently applied to the analysis of carbohydrates; discussions on analytical and preparative separations; descriptions of the principles of detection and quantitative determination of carbohydrates by the various separation techniques; reviews of sample preparations; and information on important applications.
Furthermore, the book describes in detail the different direct and indirect detection methods that have been introduced for the sensitive detection of carbohydrates. This title is useful for a wide audience including separation scientists; analytical chemists and biochemists; carbohydrate chemists; glycoprotein and glycolipid chemists; molecular biologists; and biotechnologists. The book is also a useful reference for both the experienced analyst and the newcomer and for users of modern chromatography and electrophoresis. The use of CZE in separation of C-Glycosylflavonoids by using sodium borate buffer is confirmed in this work.
Generally, CE seems to be a valid alternative in natural product studies as well as a valuable complement to other techniques. Professor E. Maluf, H. Barros, M. Frochtengarten, R. Benti and J. Oga, P. Gomes da Silva and S. Planta Med. Echeverry, G.
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